Sony MA900 Multi-Application Cell Sorter
The MA900 from Sony meets the needs of most sorting applications, supporting 12 fluorescence parameters and 4-way sorting.
Powerful, modern technologies built into the MA900 system include a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability.
Up to 14 parameters from 4 lasers
The MA900 offers choice and flexibility, enabling the detection of up to 14 parameters. Choose from 4 excitation lasers—488 nm, 405 nm, 561 nm, and 638 nm—on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and two scatter parameters.
| Startup automation ensures optimal daily alignment of the chip to lasers | With the highest level of automation available on any cell sorter, the MA900 uses sensors, software, and engineering know-how across the workflow from startup to aseptic cleaning, QC, and sort setup. Intelligent automation dramatically simplifies operations and streamlines troubleshooting. |
| Sorting that adapts to your needs with default and custom sort modes as well as 4-way sorting | Once events are gated on plots, target cells can be sorted for further analysis. Sort mode, sort gates, and sort devices can be assigned using a simple dialog box. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted population. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates. |
| Added options | If desired, the MA900 can be installed inside an optional custom class A2 level II biosafety cabinet for protection for personnel and products. The custom biosafety cabinets offered by Sony Biotechnology are designed and tested by their manufacturers using microbiological assays with the MA900 in the work area. The cabinets meet international standards including the National Sanitation Foundation Standard 49 (NSF49) and the European Standard 12469.
The cabinets incorporate a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation maximize protection. |
Optics
| Excitation lasers | 488 nm, 638 nm, 405 nm, 561 nm |
| Output power | Optical fiber output : 405 nm: (10 mW max.), 488 nm, 638 nm, and 561 nm: (36 mW max.) |
| Beam alignment | Dual axis optical system |
| Detection parameters | 12 fluorescence + 2 scatter |
| Pulse measurement | Height, Area, Width |
Fluidics
| Sample tube | Single, auto-loading tube |
| Tube types | 0.5-mL, 1.5-mL, 5-mL and 15-mL tubes |
| Sort Devices | 2-way tube, 4-way tube, multiwell plates, PCR plates |
| Temperature control | 5°C, 37°C (electric cooling method) |
| Agitation unit | Eccentric rotation |
| Magnetic drive | 300 rpm speed |
| Sorting chip size | 70 μm, 100 μm |
Sort Performance
| Event rate | 70,000 eps |
| Sorting speed | Automated frequency search range
Using the 70-μm sorting chip at 52 kHz and a threshold rate of 12,000 events per second, |
| Scatter resolution | 0.5 µm |
| Fluorescence resolution | <3.0% coefficient of variation (CV): PI stained CEN |
| Fluorescence sensitivity | FITC ≤95 MESF, PE ≤90 MESF |
Ancilliary
| Dimensions | W: 21.7” (55 cm) x D: 21.7” (55 cm) x H: 28.4” (72 cm) |
| Fluidics cart | W: 33.9” (86 cm) x D: 17.3” (44 cm) x H: 11.8” (30 cm) |
| Weight | 231 lb (105 kg) |
| Fluidics cart | 108 lb (49 kg) (dry weight) |
| LCD panel | 7-inch, 800 x 480 pixels |
| Power supply | 100–240 V, 50/60 Hz |
| Power consumption | 600 W (max.) |
| Operating temperature | 19.5°C to 27.5°C |
| Relative humidity | 20% to 80% |
Compliance
| Operating system | Microsoft® Windows® 10 Professional, 64 bit |
| Data file structure | Flow Cytometry Standard (FCS) 3.0 or 3.1 |
| Safety Standards Compliance | UL, CE, CSA |
Filter Guide
- Features & Benefits
-
Startup automation ensures optimal daily alignment of the chip to lasers With the highest level of automation available on any cell sorter, the MA900 uses sensors, software, and engineering know-how across the workflow from startup to aseptic cleaning, QC, and sort setup. Intelligent automation dramatically simplifies operations and streamlines troubleshooting. Sorting that adapts to your needs with default and custom sort modes as well as 4-way sorting Once events are gated on plots, target cells can be sorted for further analysis. Sort mode, sort gates, and sort devices can be assigned using a simple dialog box. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted population. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates. Added options If desired, the MA900 can be installed inside an optional custom class A2 level II biosafety cabinet for protection for personnel and products. The custom biosafety cabinets offered by Sony Biotechnology are designed and tested by their manufacturers using microbiological assays with the MA900 in the work area. The cabinets meet international standards including the National Sanitation Foundation Standard 49 (NSF49) and the European Standard 12469. The cabinets incorporate a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation maximize protection.
- Specifications
-
Optics
Excitation lasers 488 nm, 638 nm, 405 nm, 561 nm Output power Optical fiber output : 405 nm: (10 mW max.), 488 nm, 638 nm, and 561 nm: (36 mW max.) Beam alignment Dual axis optical system Detection parameters 12 fluorescence + 2 scatter Pulse measurement Height, Area, Width Fluidics
Sample tube Single, auto-loading tube Tube types 0.5-mL, 1.5-mL, 5-mL and 15-mL tubes Sort Devices 2-way tube, 4-way tube, multiwell plates, PCR plates Temperature control 5°C, 37°C (electric cooling method) Agitation unit Eccentric rotation Magnetic drive 300 rpm speed Sorting chip size 70 μm, 100 μm Sort Performance
Event rate 70,000 eps Sorting speed Automated frequency search range - 70 μm : 40 kHz to 52 kHz
- 100 μm [Targeted] setting : 21 kHz to 23.5 kHz
- 100 μm [Standard] setting : 27 kHz to 31 kHz
- 130 μm : 10 kHz to 12 kHz
Using the 70-μm sorting chip at 52 kHz and a threshold rate of 12,000 events per second,
purity >98% and recovery >80% can be achieved. The yield obtained is based on Poisson’s statistics. Higher threshold events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.Scatter resolution 0.5 µm Fluorescence resolution <3.0% coefficient of variation (CV): PI stained CEN Fluorescence sensitivity FITC ≤95 MESF, PE ≤90 MESF Ancilliary
Dimensions W: 21.7” (55 cm) x D: 21.7” (55 cm) x H: 28.4” (72 cm) Fluidics cart W: 33.9” (86 cm) x D: 17.3” (44 cm) x H: 11.8” (30 cm) Weight 231 lb (105 kg) Fluidics cart 108 lb (49 kg) (dry weight) LCD panel 7-inch, 800 x 480 pixels Power supply 100–240 V, 50/60 Hz Power consumption 600 W (max.) Operating temperature 19.5°C to 27.5°C Relative humidity 20% to 80% Compliance
Operating system Microsoft® Windows® 10 Professional, 64 bit Data file structure Flow Cytometry Standard (FCS) 3.0 or 3.1 Safety Standards Compliance UL, CE, CSA Filter Guide
- Documents